Journal: Nature Communications

Article Title: Deficiency in endocannabinoid synthase DAGLB contributes to early onset Parkinsonism and murine nigral dopaminergic neuron dysfunction

doi: 10.1038/s41467-022-31168-9

Figure Lengend Snippet: a Co-staining of CB1 (green), DRD1 (red), and TH (purple) in the midbrain sections of homozygous floxed CB1 ( Cnr1 fl/fl ) and Drd1-Cre/ Cnr1 fl/fl mice. Scale bar: 100 μm. More than five independent experiments were performed. b Rotarod locomotor skill training of Cnr1 fl/fl ( n = 14, 7 M/7F) and Drd1-Cre/ Cnr1 fl/fl ( n = 12, 6M/6F) mice. Data were presented as mean ± SEM. Two-way ANOVA, genotype: F (1, 24) = 6.301, p = 0.0192. c Box and whiskers plot (the minimum, the 25th percentile, the median, the 75th percentile, and the maximum) of rotarod performance in the first and 10th trial on each day with the same cohorts of Cnr1 fl/fl ( n = 14, 7M/7F) and Drd1-Cre/ Cnr1 fl/fl ( n = 12, 6M/6F) mice. Multiple unpaired t test, two-tailed, * p < 0.05, ** p < 0.005.

Article Snippet: The antibodies used for immunostaining included rat monoclonal anti-DRD1 (Sigma-Aldrich, D2944, clone 1-1-F11 s.E6, 1:500), mouse monoclonal anti-CB1 (Synaptic systems, 258011, clone 289c1, 1:500), rabbit polyclonal anti-TH (Pel-Freez Biologicals, P40101, 1:2500), mouse monoclonal anti-TH (ImmunoStar, 22941, 1:1000), chicken polyclonal anti-TH (Aves Labs, TYH, 1:500), chicken polyclonal anti-GFP (Aves Labs, GFP-1020, 1:1000), rabbit polyclonal anti-RFP (Rockland, 600-401-379, 1:1000), rabbit monoclonal anti-HA-Tag (Cell Signaling, 3724 S, clone 29F4, 1:100), guinea pig polyclonal anti-NeuN (Synaptic systems, 266 004, 1:1000), rabbit polyclonal anti-Iba1 (Wako, 019-19741, 1:1000), and mouse monoclonal anti-TOM20 (Santa Cruz, sc-17764, clone F-10, 1:200).

Techniques: Staining, Two Tailed Test

Journal: Psychopharmacology

Article Title: Extracellular Dopamine, Acetylcholine, and Activation of Dopamine D1 and D2 Receptors after Selective Breeding for Cocaine Self-Administration in Rats

doi: 10.1007/s00213-017-4640-7

Figure Lengend Snippet: Counts of cells expressing c-Fos, D1R and D2R in different brain regions of LS and HS rats. Values indicate the number of cells per field, scored under blinded conditions, with data shown for five animals per condition.

Article Snippet: Sections were then incubated with sheep anti-c-Fos (1:500, CBL440, Millipore, Temecula, USA), combined with either mouse anti-Dopamine D1R (1:1000, NB110, Novus Biologicals, Littleton, USA) or rabbit anti-Dopamine D2R (1:500, AB5084P, Millipore, Temecula, USA) antibodies over 48 h in a humidi ed chamber at 4°.

Techniques: Expressing

Journal: Brain, behavior, and immunity

Article Title: HIV-1 Tat protein enhances sensitization to methamphetamine by affecting dopaminergic function

doi: 10.1016/j.bbi.2017.05.004

Figure Lengend Snippet: Immunohistochemistry on paraffin embedded sections was utilized examine the protein distribution and levels of dopamine receptor D1 (A, B, C, D), dopamine receptor D2 (E, F, G, H), as well as of IBA-1 (I, J, K, L) in SAL TAT− (A, E, I), SAL TAT+ (B, F, J), METH TAT− (C, G, K), and METH TAT+ (D, H, L) mice. Representative positive cells in the 40× magnification images were labeled with a black arrow. (M) Normalized intensity density was calculated in ImageJ. Data are expressed as Mean ± SEM (n=5). * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Sections were blocked with 5g/l Casein (Sigma Aldrich) in PBS, containing 0.5g/l Thimerosal (Sigma Aldrich) and incubated with Iba-1 antibody (Wako Lab Chemicals, Richmond, VA), the anti-mouse DRD1 antibody (NLS43, Novus Biologicals, Littleton, CO), or anti mouse DRD2 (orb154598, Biorbyt, San Francisco, CA), each one diluted in Casein buffer.

Techniques: Immunohistochemistry, Labeling

Journal: Journal of Translational Medicine

Article Title: Taltirelin induces TH expression by regulating TRHR and RARα in medium spiny neurons

doi: 10.1186/s12967-024-06020-x

Figure Lengend Snippet: TRHR and TH mainly colocalize GABAergic neurons. A Immunofluorescence colocalization of TRHR with DRD1 and DRD2 in the Lesioned striatum of TAL-treated Hemi-PD rats. Scale bar: 20 μm. B, C, D Scatter plots and Pearson correlation coefficients of colocalization

Article Snippet: Primary antibodies against the following targets were used: TH (Santa Cruz Biotechnology, sc-25269, 1:2000), TRHR (Novus Biologicals, NBP2-24726, 1:1000), ERK1/2 (Cell Signaling Technology, 4695 s, 1:4000), P-ERK1/2 (Cell Signaling Technology, 4370 s, 1:2000), GABA (Abcam, ab86186, 1:8000), RARα (ABclonal, A0370, 1:8000), DRD1 (Novus Biologicals, NB110-60017, 1:1000), DRD2 (ABclonal, A12930, 1:1000; Novus Biologicals, MAB9266, 1:1000), and GAPDH (Proteintech, 60,004–1-Ig, 1:8000).

Techniques: Immunofluorescence